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Electrochemical nanosensor for real-time direct imaging of nitric oxide in living brain

Title
Electrochemical nanosensor for real-time direct imaging of nitric oxide in living brain
Authors
Jo A.Do H.Jhon G.-J.Suh M.Lee Y.
Ewha Authors
전길자이영미
SCOPUS Author ID
전길자scopus; 이영미scopus
Issue Date
2011
Journal Title
Analytical Chemistry
ISSN
0003-2700JCR Link
Citation
vol. 83, no. 21, pp. 8314 - 8319
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
As gaseous nitric oxide (NO), a critical and multifaceted biomarker, diffuses easily once released, identifying the precise sources of NO release is a challenge. This study developed a new technique for real-time in vivo direct NO imaging by coupling an amperometric NO nanosensor with scanning electrochemical microscopy. This technique provides three-dimensional information of the NO releasing sites in an intact living mouse brain with high sensitivity and spatial resolution. Immunohistochemical analysis was carried out to confirm the anatomical reliability of the acquired electrochemical NO image. The real-time NO imaging results were well matched with the corresponding immunohistochemical analysis of neuronal NO synthase immunoreactive (nNOS-IR) cells, i.e., NO releasing sites in a living brain. The imaged NO local concentrations were confirmed to be closely related to the location in depth, the size of the nNOS-IR cell, and the intensity of nNOS immunoreactivity. This paper demonstrates the first direct electrochemical NO imaging of a living brain. © 2011 American Chemical Society.
DOI
10.1021/ac202225n
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자연과학대학 > 화학·나노과학전공 > Journal papers
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