Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2016-08-28T12:08:06Z | - |
dc.date.available | 2016-08-28T12:08:06Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.other | OAK-7860 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221862 | - |
dc.description.abstract | Rapamycin is a macrocyclic polyketide with immunosuppressive, antifungal, and anticancer activity produced by Streptomyces hygroscopicus ATCC 29253. Rapamycin production by a mutant strain (UV2-2) induced by ultraviolet mutagenesis was improved by approximately 3.2-fold (23.6 mg/l) compared to that of the wild-type strain. The comparative analyses of gene expression and intracellular acyl-CoA pools between wild-type and the UV2-2 strains revealed that the increased production of rapamycin in UV2-2 was due to the prolonged expression of rapamycin biosynthetic genes, but a depletion of intracellular methylmalonyl-CoA limited the rapamycin biosynthesis of the UV2-2 strain. Therefore, three different metabolic pathways involved in the biosynthesis of methylmalonyl-CoA were evaluated to identify the effective precursor supply pathway that can support the high production of rapamycin: propionyl-CoA carboxylase (PCC), methylmalonyl-CoA mutase, and methylmalonyl-CoA ligase. Among them, only the PCC pathway along with supplementation of propionate was found to be effective for an increase in intracellular pool of methylmalonyl-CoA and rapamycin titers in UV2-2 strain (42.8 mg/l), indicating that the PCC pathway is a major methylmalonyl-CoA supply pathway in the rapamycin producer. These results demonstrated that the combined approach involving traditional mutagenesis and metabolic engineering could be successfully applied to the diagnosis of yield-limiting factors and the enhanced production of industrially and clinically important polyketide compounds. © 2011 Springer-Verlag. | - |
dc.language | English | - |
dc.title | A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253 | - |
dc.type | Article | - |
dc.relation.issue | 5 | - |
dc.relation.volume | 91 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1389 | - |
dc.relation.lastpage | 1397 | - |
dc.relation.journaltitle | Applied Microbiology and Biotechnology | - |
dc.identifier.doi | 10.1007/s00253-011-3348-6 | - |
dc.identifier.wosid | WOS:000293751500013 | - |
dc.identifier.scopusid | 2-s2.0-80052611955 | - |
dc.author.google | Jung W.S. | - |
dc.author.google | Yoo Y.J. | - |
dc.author.google | Park J.W. | - |
dc.author.google | Park S.R. | - |
dc.author.google | Han A.R. | - |
dc.author.google | Ban Y.H. | - |
dc.author.google | Kim E.J. | - |
dc.author.google | Kim E. | - |
dc.author.google | Yoon Y.J. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |