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Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties

Title
Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties
Authors
Oh B.N.Lee S.Park H.-Y.Baeg J.-O.Yoon M.-Y.Kim J.
Ewha Authors
김진흥
SCOPUS Author ID
김진흥scopus
Issue Date
2011
Journal Title
Analyst
ISSN
0003-2654JCR Link
Citation
vol. 136, no. 16, pp. 3384 - 3388
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A homogeneous assay of the protective antigen in anthrax toxin is reported using two new PA-specific aptamers for selective and sensitive detection, based on reduction in the fluorescence emission according to the formation of the aptamer-PA ternary complex. PA at 1 nM was readily detected using OliGreen as a fluorophore in HEPES buffer. We also demonstrated that the PA detection could be performed in blood serum. The binding interaction between the aptamer and PA was strong enough to dehybridize double-stranded DNA paired completely with 12 bases at room temperature. Moreover, this fluorescence study revealed that the binding sites of the two aptamers were located differently on the PA protein. We believe our approach may lay the groundwork for the real-time detection of PA. © 2011 The Royal Society of Chemistry.
DOI
10.1039/c0an00978d
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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