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Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry

Title
Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry
Authors
Noh K.Park J.H.Kim M.Jung M.Ha H.Kwon K.-I.Lee H.J.Kang W.
Ewha Authors
이화정하헌주
SCOPUS Author ID
이화정scopus; 하헌주scopus
Issue Date
2011
Journal Title
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085JCR Link
Citation
vol. 56, no. 1, pp. 114 - 117
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats. © 2011 Elsevier B.V.
DOI
10.1016/j.jpba.2011.04.008
Appears in Collections:
약학대학 > 약학과 > Journal papers
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