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Adenosine derived from Staphylococcus aureus-engulfed macrophages functions as a potent stimulant for the induction of inflammatory cytokines in mast cells

Title
Adenosine derived from Staphylococcus aureus-engulfed macrophages functions as a potent stimulant for the induction of inflammatory cytokines in mast cells
Authors
Ma Y.J.Kim C.-H.Ryu K.-H.Kim M.-S.So Y.-I.Lee K.-J.Garred P.Lee B.-L.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopus
Issue Date
2011
Journal Title
BMB Reports
ISSN
1976-6696JCR Link
Citation
vol. 44, no. 5, pp. 335 - 340
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
In this study, we attempted to isolate novel mast cell-stimulating molecules from Staphylococcus aureus. Water-soluble extract of S. aureus cell lysate strongly induced human interleukin-8 in human mast cell line-1 and mouse interleukin-6 in mouse bone marrow-derived mast cells. The active molecule was purified to homogeneity through a C18 reverse phase HPLC column. By determination of its structure by MALDITOF and 1H- and 13C-NMR, adenosine was revealed to be responsible for the observed cytokine induction activities. Further studies using 8-sulfophenyl theophylline, a selective adenosine receptor blocker, verified that purified adenosine can induce interleukin-8 production via adenosine receptors on mast cells. Moreover, adenosine was purified from S. aureusengulfed RAW264.7 cells, a murine macrophage cell line, used to induce phagocytosis of S. aureus. These results show a novel view of the source of exogenous adenosine in vivo and provide a mechanistic link between inflammatory disease and bacterial infection.
DOI
10.5483/BMBRep.2011.44.5.335
Appears in Collections:
약학대학 > 약학과 > Journal papers
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