View : 19 Download: 0

Design and evaluation of variants of the protein transduction domain originated from translationally controlled tumor protein

Title
Design and evaluation of variants of the protein transduction domain originated from translationally controlled tumor protein
Authors
Kim M.Maeng J.Jung J.Kim H.Y.Kim H.-J.Kwon Y.Lee K.
Ewha Authors
김화정이경림권영주맹지혜
SCOPUS Author ID
김화정scopus; 이경림scopus; 권영주scopus; 맹지혜scopus
Issue Date
2011
Journal Title
European Journal of Pharmaceutical Sciences
ISSN
0928-0987JCR Link
Citation
vol. 43, no. 41276, pp. 25 - 31
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Protein transduction domains (PTDs) have been successfully employed to deliver therapeutic cargos both in vitro and in vivo because of their cellular penetrating ability. We previously reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of human translationally controlled tumor protein (TCTP) functions as a PTD. TCTP-PTD is quite different from other well-known PTDs in its hydrophobic composition and structural character, and the sequence requirements for transduction remain unknown. To identify the role of each residue, we compared the cellular uptake of various deletion mutants and Ala substituents of TCTP-PTD. The results showed that the amino terminal residues and the hydrophobic nature of the peptide, with a minimal length of nine residues, were necessary for transduction. Based on the elucidated sequence requirements, we designed and evaluated variants to improve the efficiency and solubility through sequential modification of TCTP-PTD. During the optimization process, we also delineated the contribution of residues and the advantageous composition of sequences for cellular uptake. © 2011 Elsevier B.V. All rights reserved.
DOI
10.1016/j.ejps.2011.03.007
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE