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Proteomic identification of radiation response markers in mouse intestine and brain

Title
Proteomic identification of radiation response markers in mouse intestine and brain
Authors
Lim Y.-B.Pyun B.-J.Lee H.-J.Jeon S.-R.Jin Y.B.Lee Y.-S.
Ewha Authors
이윤실
SCOPUS Author ID
이윤실scopus
Issue Date
2011
Journal Title
Proteomics
ISSN
1615-9853JCR Link
Citation
vol. 11, no. 7, pp. 1254 - 1263
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Increasing efforts are being made to develop more sensitive and faster molecular methodologies at the genomic and proteomic levels for the identification of protein markers after exposure to ionizing radiation (IR). However, few specific protein markers, especially organ-specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR-treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2-DE data. These were heat shock protein 5 (HSP 5), HSP 90kDa β, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue-specific protein markers of IR exposure. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI
10.1002/pmic.201000332
Appears in Collections:
약학대학 > 약학과 > Journal papers
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