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Prominent bone loss mediated by RANKL and IL-17 produced by CD4+ T cells in tallyho/JngJ mice

Title
Prominent bone loss mediated by RANKL and IL-17 produced by CD4+ T cells in tallyho/JngJ mice
Authors
Won H.Y.Lee J.-A.Park Z.S.Song J.S.Kim H.Y.Jang S.-M.Yoo S.-E.Rhee Y.Hwang E.S.Bae M.A.
Ewha Authors
황은숙
SCOPUS Author ID
황은숙scopus
Issue Date
2011
Journal Title
PLoS ONE
ISSN
1932-6203JCR Link
Citation
vol. 6, no. 3
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Increasing evidence that decreased bone density and increased rates of bone fracture are associated with abnormal metabolic states such as hyperglycemia and insulin resistance indicates that diabetes is a risk factor for osteoporosis. In this study, we observed that TallyHo/JngJ (TH) mice, a polygenic model of type II diabetes, spontaneously developed bone deformities with osteoporotic features. Female and male TH mice significantly gained more body weight than control C57BL/6 mice upon aging. Interestingly, bone density was considerably decreased in male TH mice, which displayed hyperglycemia. The osteoblast-specific bone forming markers osteocalcin and osteoprotegerin were decreased in TH mice, whereas osteoclast-driven bone resorption markers such as IL-6 and RANKL were significantly elevated in the bone marrow and blood of TH mice. In addition, RANKL expression was prominently increased in CD4+ T cells of TH mice upon T cell receptor stimulation, which was in accordance with enhanced IL-17 production. IL-17 production in CD4+ T cells was directly promoted by treatment with leptin while IFN-γ production was not. Moreover, blockade of IFN-γ further increased RANKL expression and IL-17 production in TH-CD4+ T cells. In addition, the osteoporotic phenotype of TH mice was improved by treatment with alendronate. These results strongly indicate that increased leptin in TH mice may act in conjunction with IL-6 to preferentially stimulate IL-17 production in CD4+ T cells and induce RANKL-mediated osteoclastogenesis. Accordingly, we propose that TH mice could constitute a beneficial model for osteoporosis. © 2011 Won et al.
DOI
10.1371/journal.pone.0018168
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약학대학 > 약학과 > Journal papers
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