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Effects of synthetic pseudoceramides on sphingosine kinase activity in F9-12 cells
- Effects of synthetic pseudoceramides on sphingosine kinase activity in F9-12 cells
- Jin Y.-X.; Shin K.-O.; Park M.-Y.; Lee S.-H.; Park B.-D.; Oh S.; Yoo H.-S.; Lee Y.-M.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Biomolecules and Therapeutics
- vol. 19, no. 1, pp. 134 - 139
- SCIE; SCOPUS
- Sphingosine kinase (SPHK) has a central role to control cell death and cell proliferation, which is suggested as a sphingolipid rheostat by regulating the levels between ceramide and sphingosine 1-phosphate (S1P). Therefore, physiological regulators of SPHK will be a good candidate to develop a new targeted drug. For this purpose, a series of synthetic pseudoceramides were tested by SPHK assay either cell-based or cell-free system. K10PC-5 strongly inhibited SPHK, while K6PC-5 activated SPHK in cell-free system. Specifically, K6PC-5 activated SPHK under the co-treatment with 50 μM dimethylsphingosine (DMS), a SPHK inhibitor. Collectively, we developed a simple SPHK assay system to find SPHK regulatory pseudoceramide compounds, K10PC-5 and K6PC-5 which may be useful to cancer treatment or immune regulation like FTY720, a synthetic sphingolipid mimetic compound. © 2011 The Korean Society of Applied Pharmacology.
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