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The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells

Title
The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells
Authors
Lee J.-Y.Nam H.Park Y.-J.Lee S.-J.Chung C.-P.Han S.-B.Lee G.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2011
Journal Title
In Vitro Cellular and Developmental Biology - Animal
ISSN
1071-2690JCR Link
Citation
vol. 47, no. 2, pp. 157 - 164
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Platelet-rich plasma (PRP) is an emerging therapeutic application because PRP contains various growth factors that have beneficial effects on tissue regeneration and engineering. Mesenchymal stem cells and PRP derived from peripheral blood have been well studied. In this study, we investigated the effects of PRP derived from human umbilical cord blood (UCB-PRP) on proliferation, alkaline phosphatase (ALP) activity, and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and periodontal ligament stem cells (PDLSCs). Three types of dental stem cells were primarily isolated and characterized by flow cytometric analysis. Dental stem cells were exposed to various concentrations of UCB-PRP, which resulted in the proliferation of dental stem cells. Treatment with 2% UCB-PRP resulted in the highest level of proliferation. The ALP activity of DPSCs and PDLSCs increased following treatment with UCB-PRP in a dose-dependent manner up to a concentration of 2%. ALP activity decreased with higher concentration of UCB-PRP. The effects of UCB-PRP on calcium deposition were similar to those on proliferation and ALP activity. Treatment with 2% UCB-PRP resulted in the highest calcium depositions in DPSCs and PDLSCs; however, treatment with 1% UCB-PRP resulted in the highest calcium deposition in SHEDs. The concentrations of platelet-derived growth factor-AB and transforming growth factor-β1 in UCB-PRP were investigated and found to be comparable to the amounts in peripheral blood. Overall, UCB-PRP had beneficial effects on the proliferation and osteogenic differentiation of dental stem cells. Determination of the optimal concentration of UCB-PRP requires further investigation for clinical applications. © 2010 The Society for In Vitro Biology.
DOI
10.1007/s11626-010-9364-5
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약학대학 > 약학과 > Journal papers
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