Post-translational his-cys cross-linkage formation in tyrosinase induced by copper(II)-peroxo species

Abstract

Autocatalytic formation of His-Cys cross-linkage in the enzyme active site of tyrosinase from Aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with CuII under aerobic conditions, where a (μ-η2:η2-peroxo)dicopper(II) species has been suggested to be involved as a key reactive intermediate. © 2011 American Chemical Society.