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Intracellular drug delivery of layered double hydroxide nanoparticles

Title
Intracellular drug delivery of layered double hydroxide nanoparticles
Authors
Oh J.-M.Park C.-B.Choy J.-H.
Ewha Authors
최진호
SCOPUS Author ID
최진호scopus
Issue Date
2011
Journal Title
Journal of Nanoscience and Nanotechnology
ISSN
1533-4880JCR Link
Citation
vol. 11, no. 2, pp. 1632 - 1635
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Intracellular drug delivery of layered double hydroxide (LDH) nanocarriers have been examined in human osteosarcoma Saos-2 cell culture line by both electron and confocal microscopies. For transmission electron microsopic (TEM) study, LDHs and anticancer drug, methotrexate (MTX) loaded LDHs were synthesized and the particle size was controlled. From the scanning electron microscopic (SEM) studies, morphologies of LDH nanoparticle and its MTX intercalated form were proven to be platelike hexagonal with an average size of ∼150 nm. In order to understand the cellular penetration behavior, both nanoparticles were treated to human osteosarcoma Saos-2 cell culture lines and the cellular uptake pattern with respect to incubation time was observed by TEM and SEM. We observed that the nanoparticles are attached at the cellular membrane at first and then internalized into the cells via endocytosis within 1 h. Then are located in the intracellular vacuole (endosome). In order to examine the intracellular drug delivery mechanism of LDH nanoparticles, fluorescein 5-isothiocyanate (FITC) labeled MTX was intercalated into LDH and treated on Saos-2 cells. Laser scanning confocal microscopic studies revealed that the FITC-MTX molecules were first internalized with LDH nanocarriers via endocytosis, and located in endosome to deliver loaded drug to target cellular organ. It was, therefore, concluded that LDH could play a role as drug delivery nanocarriers. Copyright © 2011 American Scientific Publishers All rights reserved.
DOI
10.1166/jnn.2011.3409
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자연과학대학 > 화학·나노과학전공 > Journal papers
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