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Screening and characterization of high-affinity ssDNA aptamers against anthrax protective antigen

Title
Screening and characterization of high-affinity ssDNA aptamers against anthrax protective antigen
Authors
Choi J.S.Kim S.G.Lahousse M.Park H.-Y.Park H.-C.Jeong B.Kim J.Kim S.-K.Yoon M.-Y.
Ewha Authors
정병문김진흥
SCOPUS Author ID
정병문scopus; 김진흥scopus
Issue Date
2011
Journal Title
Journal of Biomolecular Screening
ISSN
1087-0571JCR Link
Citation
vol. 16, no. 2, pp. 266 - 271
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The protective antigen (PA) of Bacillus anthracis is a secreted protein that functions as a critical virulence factor. Protective antigen has been selected as a biomarker in detecting bacterial infection. The in vitro selection method, systematic evolution of ligands by exponential enrichment (SELEX), was used to find single-stranded DNAs that were tightly bound to PA. After 8 rounds of the SELEX process with PA, 4 different oligonucleotides (referred to as aptamers) that contain a 30-residue ssDNA sequence were identified. Dissociation constant (K d) values with Cy3-attached aptamers were determined via fluorophotometry to be within a nanomolar range. The authors attempted to visualize the detection of PA using an aptamer-based enzyme-linked immunosorbent assay method, which has proven to be successful within a nanomolar K d value range. Furthermore, 2 of the 4 aptamers exhibited specificity to PA against bovine serum albumin and bovine serum. The results of this study demonstrate the analytical potential of an oligonucleotide-based biosensor for a wide variety of applications, particularly in diagnosing disease through specific protein biomarkers. © 2011 Society for Laboratory Automation and Screening.
DOI
10.1177/1087057110391787
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자연과학대학 > 화학·나노과학전공 > Journal papers
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