Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 하헌주 | * |
dc.date.accessioned | 2016-08-28T12:08:04Z | - |
dc.date.available | 2016-08-28T12:08:04Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 1226-4512 | * |
dc.identifier.other | OAK-7228 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221312 | - |
dc.description.abstract | Excessive extracellular matrix (ECM) accumulation is the main feature of chronic renal disease including diabetic nephropathy. Plasminogen activator inhibitor (PAI)-1 is known to play an important role in renal ECM accumulation in part through suppression of plasmin generation and matrix metalloproteinase (MMP) activation. The present study examined the effect of PAI-1 antisense oligodeoxynucleotide (ODN) on fibronectin upregulation and plasmin/MMP suppression in primary mesangial cells cultured under high glucose (HG) or transforming growth factor (TGF)-β 1, major mediators of diabetic renal ECM accumulation. Growth arrested and synchronized rat primary mesangial cells were transfected with 1 μM phosphorothioate-modified antisense or control mis-match ODN for 24 hours with cationic liposome and then stimulated with 30 mM D-glucose or 2 ng/ml TGF-β 1. PAI-1 or fibronectin protein was measured by Western blot analysis. Plasmin activity was determined using a synthetic fluorometric plasmin substrate and MMP-2 activity analyzed using zymography. HG and TGF-β 1 significantly increased PAI-1 and fibronectin protein expression as well as decreased plasmin and MMP-2 activity. Transient transfection of mesangial cells with PAI-1 antisense ODN, but not mis-match ODN, effectively reversed basal as well as HG- and TGF-β 1-induced suppression of plasmin and MMP-2 activity. Both basal and upregulated fibronectin secretion were also inhibited by PAI-1 antisense ODN. These data confirm that PAI-1 plays an important role in ECM accumulation in diabetic mesangium through suppression of protease activity and suggest that PAI-1 antisense ODN would be an effective therapeutic strategy for prevention of renal fibrosis including diabetic nephropathy. | * |
dc.language | English | * |
dc.title | Plasminogen activator inhibitor-1 antisense oligodeoxynucleotides abrogate mesangial fibronectin accumulation | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 14 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 385 | * |
dc.relation.lastpage | 390 | * |
dc.relation.journaltitle | Korean Journal of Physiology and Pharmacology | * |
dc.identifier.doi | 10.4196/kjpp.2010.14.6.385 | * |
dc.identifier.wosid | WOS:000286303100006 | * |
dc.identifier.scopusid | 2-s2.0-79251518632 | * |
dc.author.google | Park J. | * |
dc.author.google | Seo J.Y. | * |
dc.author.google | Ha H. | * |
dc.contributor.scopusid | 하헌주(7202277106) | * |
dc.date.modifydate | 20240422113229 | * |