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Parallel Analysis of 7 Food-borne Pathogens using capillary electrophoresis-based single-strand conformation polymorphism
- Title
- Parallel Analysis of 7 Food-borne Pathogens using capillary electrophoresis-based single-strand conformation polymorphism
- Authors
- Kim A.-R.; Oh M.-H.; Seol K.-H.; Shin G.-W.; Jung G.Y.; Oh S.
- Ewha Authors
- 오상석
- SCOPUS Author ID
- 오상석
- Issue Date
- 2010
- Journal Title
- Food Science and Biotechnology
- ISSN
- 1226-7708
- Citation
- Food Science and Biotechnology vol. 19, no. 6, pp. 1441 - 1447
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) coupled with multiplex polymerase chain reaction (PCR) method was used for the detection of 7 pathogens associated with foodborne illness, including Salmonella enterica, Clostridium perfringens, Bacillus cereus, Listeria monocytogenes, Yersinia enterocolitica, Vibrio parahaemolyticus, and Escherichia coli O157:H7. The method was applied to model food systems, both of culture medium and cooked rice. The detection limit of individual microbes was in the range of 10 1-10 3 CFU/g, and that of the mixture of 7 microbes was 10 3 CFU/g in the cooked rice sample. This method allowed the detection and identification of all 7 food-borne pathogens within 5 hr without the requirement for enrichment steps. © KoSFoST and Springer 2010.
- DOI
- 10.1007/s10068-010-0206-8
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
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