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Parallel Analysis of 7 Food-borne Pathogens using capillary electrophoresis-based single-strand conformation polymorphism

Title
Parallel Analysis of 7 Food-borne Pathogens using capillary electrophoresis-based single-strand conformation polymorphism
Authors
Kim A.-R.Oh M.-H.Seol K.-H.Shin G.-W.Jung G.Y.Oh S.
Ewha Authors
오상석
SCOPUS Author ID
오상석scopus
Issue Date
2010
Journal Title
Food Science and Biotechnology
ISSN
1226-7708JCR Link
Citation
vol. 19, no. 6, pp. 1441 - 1447
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) coupled with multiplex polymerase chain reaction (PCR) method was used for the detection of 7 pathogens associated with foodborne illness, including Salmonella enterica, Clostridium perfringens, Bacillus cereus, Listeria monocytogenes, Yersinia enterocolitica, Vibrio parahaemolyticus, and Escherichia coli O157:H7. The method was applied to model food systems, both of culture medium and cooked rice. The detection limit of individual microbes was in the range of 10 1-10 3 CFU/g, and that of the mixture of 7 microbes was 10 3 CFU/g in the cooked rice sample. This method allowed the detection and identification of all 7 food-borne pathogens within 5 hr without the requirement for enrichment steps. © KoSFoST and Springer 2010.
DOI
10.1007/s10068-010-0206-8
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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