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Parallel Analysis of 7 Food-borne Pathogens using capillary electrophoresis-based single-strand conformation polymorphism
- Parallel Analysis of 7 Food-borne Pathogens using capillary electrophoresis-based single-strand conformation polymorphism
- Kim A.-R.; Oh M.-H.; Seol K.-H.; Shin G.-W.; Jung G.Y.; Oh S.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Food Science and Biotechnology
- vol. 19, no. 6, pp. 1441 - 1447
- SCIE; SCOPUS; KCI
- Capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) coupled with multiplex polymerase chain reaction (PCR) method was used for the detection of 7 pathogens associated with foodborne illness, including Salmonella enterica, Clostridium perfringens, Bacillus cereus, Listeria monocytogenes, Yersinia enterocolitica, Vibrio parahaemolyticus, and Escherichia coli O157:H7. The method was applied to model food systems, both of culture medium and cooked rice. The detection limit of individual microbes was in the range of 10 1-10 3 CFU/g, and that of the mixture of 7 microbes was 10 3 CFU/g in the cooked rice sample. This method allowed the detection and identification of all 7 food-borne pathogens within 5 hr without the requirement for enrichment steps. © KoSFoST and Springer 2010.
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