View : 19 Download: 0

Nonviral gene delivery to human ovarian cancer cells using arginine-grafted PAMAM dendrimer

Title
Nonviral gene delivery to human ovarian cancer cells using arginine-grafted PAMAM dendrimer
Authors
Jang S.H.Choi S.J.Oh J.H.Chae S.W.Nam K.Park J.S.Lee H.J.
Ewha Authors
이화정
SCOPUS Author ID
이화정scopus
Issue Date
2011
Journal Title
Drug Development and Industrial Pharmacy
ISSN
0363-9045JCR Link
Citation
vol. 37, no. 1, pp. 41 - 46
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Background: A specific and effective strategy is in demand to treat ovarian cancer successfully. Epidermal growth factor receptor (EGFR) is highly expressed in ovarian cancer, and thus EGFR antisense gene therapy can be a potential therapeutic strategy. Method: l-Arginine-grafted-polyamidoamine dendrimer (PAMAM-Arg) has been reported to be a novel nonviral gene delivery carrier. Therefore, the ability of PAMAM-Arg in transferring a luciferase gene to ovarian carcinoma SK-OV3 cells has been examined, and the cytotoxicity of the cationic polymer has been investigated. In addition, the suppression of cell proliferation has been evaluated by transferring an EGFR antisense gene to SK-OV3 cells using PAMAM-Arg. Polyethyleneimine (PEI) 25K was used as a positive control. Results: As a result, in vitro gene transfection efficiency of PAMAM-Arg was enhanced with increasing transfection time and NP ratios. PAMAM-Arg transferred the luciferase gene into cells more efficiently than PEI. In addition, PAMAM-Arg was minimally toxic to the cells whereas PEI 25K was highly toxic. The polyplexes formed by the EGFR antisense gene and PAMAM-Arg significantly reduced thymidine incorporation into the cells suggesting the suppression of cancer cell proliferation. Conclusion: These results suggest that a PAMAM-ArgEGFR antisense gene complex can be used as a safe and efficient therapeutic agent for cancer gene therapy. © 2011 Informa UK, Ltd.
DOI
10.3109/03639045.2010.489563
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE