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The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis
- The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis
- Moon J.Y.; Mosaddik A.; Kim H.; Cho M.; Choi H.-K.; Kim Y.S.; Cho S.K.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Food Chemistry
- vol. 125, no. 2, pp. 369 - 375
- SCI; SCIE; SCOPUS
- The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 μg/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 μg/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3′, 4′, 5′ trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer. © 2010 Elsevier Ltd.
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