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Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry

Title
Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry
Authors
Noh K.Kim S.Y.Kam Y.L.Choo H.-Y.P.Lee H.J.Kang W.
Ewha Authors
박혜영이화정
SCOPUS Author ID
박혜영scopus; 이화정scopus
Issue Date
2011
Journal Title
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085JCR Link
Citation
vol. 54, no. 3, pp. 568 - 571
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A novel T-type calcium channel blocker, 4-amino-1-{4-[(4-chloro-phenyl)-phenyl-methyl]-piperazin-1-yl}-butan-1-one (HYP-10) has been synthesized, and the compound has shown promise as both a nociceptive and inflammatory pain reliever as well as an analgesic in a rat neuropathic pain model. A quantification method was developed for the determination of HYP-10 in rat plasma. After simple protein precipitation with methanol, HYP-10 and the internal standard, methaqualone were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma HYP-10 concentration after a single intravenous administration of the compound in rats. © Elsevier B.V.
DOI
10.1016/j.jpba.2010.10.007
Appears in Collections:
약학대학 > 약학과 > Journal papers
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