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Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells

Title
Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells
Authors
Moon U.Y.Bae J.H.Kim C.-H.Kim H.J.Kang J.W.Yoon J.-H.
Ewha Authors
배정호
SCOPUS Author ID
배정호scopus
Issue Date
2010
Journal Title
Journal of Cellular Biochemistry
ISSN
0730-2312JCR Link
Citation
vol. 111, no. 2, pp. 476 - 487
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Lysozyme is a major component of airway epithelial secretions, acts as cationic anti-microbial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen-activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that overexpression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase-c-Myb signaling pathways in NCI-H292 cells. © 2010 Wiley-Liss, Inc.
DOI
10.1002/jcb.22730
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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