Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배정호 | * |
dc.date.accessioned | 2016-08-28T12:08:45Z | - |
dc.date.available | 2016-08-28T12:08:45Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0730-2312 | * |
dc.identifier.other | OAK-6947 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221081 | - |
dc.description.abstract | Lysozyme is a major component of airway epithelial secretions, acts as cationic anti-microbial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen-activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that overexpression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase-c-Myb signaling pathways in NCI-H292 cells. © 2010 Wiley-Liss, Inc. | * |
dc.language | English | * |
dc.title | Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 111 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 476 | * |
dc.relation.lastpage | 487 | * |
dc.relation.journaltitle | Journal of Cellular Biochemistry | * |
dc.identifier.doi | 10.1002/jcb.22730 | * |
dc.identifier.wosid | WOS:000282482400024 | * |
dc.identifier.scopusid | 2-s2.0-77957279133 | * |
dc.author.google | Moon U.Y. | * |
dc.author.google | Bae J.H. | * |
dc.author.google | Kim C.-H. | * |
dc.author.google | Kim H.J. | * |
dc.author.google | Kang J.W. | * |
dc.author.google | Yoon J.-H. | * |
dc.contributor.scopusid | 배정호(35168533900;57199910352) | * |
dc.date.modifydate | 20231120162154 | * |