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dc.contributor.author배정호*
dc.date.accessioned2016-08-28T12:08:45Z-
dc.date.available2016-08-28T12:08:45Z-
dc.date.issued2010*
dc.identifier.issn0730-2312*
dc.identifier.otherOAK-6947*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/221081-
dc.description.abstractLysozyme is a major component of airway epithelial secretions, acts as cationic anti-microbial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen-activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that overexpression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase-c-Myb signaling pathways in NCI-H292 cells. © 2010 Wiley-Liss, Inc.*
dc.languageEnglish*
dc.titleActivation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells*
dc.typeArticle*
dc.relation.issue2*
dc.relation.volume111*
dc.relation.indexSCI*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.startpage476*
dc.relation.lastpage487*
dc.relation.journaltitleJournal of Cellular Biochemistry*
dc.identifier.doi10.1002/jcb.22730*
dc.identifier.wosidWOS:000282482400024*
dc.identifier.scopusid2-s2.0-77957279133*
dc.author.googleMoon U.Y.*
dc.author.googleBae J.H.*
dc.author.googleKim C.-H.*
dc.author.googleKim H.J.*
dc.author.googleKang J.W.*
dc.author.googleYoon J.-H.*
dc.contributor.scopusid배정호(35168533900;57199910352)*
dc.date.modifydate20231120162154*
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의과대학 > 의학과 > Journal papers
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