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Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells

Title
Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells
Authors
Kim S.-J.Jung H.-J.Hyun D.-H.Park E.-H.Kim Y.-M.Lim C.-J.
Ewha Authors
현동훈
SCOPUS Author ID
현동훈scopus
Issue Date
2010
Journal Title
Biochimie
ISSN
0300-9084JCR Link
Citation
vol. 92, no. 8, pp. 927 - 932
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The cellular roles of glutathione reductase (GR) in the reactive oxygen species (ROS)-induced apoptosis were studied using the HepG2 cells transfected with GR. The overexpression of GR caused a marked enhancement in reduced and oxidized glutathione (GSH/GSSG) ratio, and significantly decreased ROS levels in the stable transfectants. Hydrogen peroxide (H 2O 2), under the optimal condition for apoptosis, significantly decreased cellular viability and total GSH content, and rather increased ROS level, apoptotic percentage and caspase-3 activity in the mock-transfected cells. However, hydrogen peroxide could not largely generate these apoptotic changes in cellular viability, ROS level, apoptotic percentage, caspase-3 activity and total GSH content in the cells overexpressing GR. Taken together, GR may play a protective role against oxidative stress. © 2010 Elsevier Masson SAS.
DOI
10.1016/j.biochi.2010.03.007
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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