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Hip2 interacts with and destabilizes Smac/DIABLO

Title
Hip2 interacts with and destabilizes Smac/DIABLO
Authors
Bae Y.Kho C.W.Lee S.Y.Rhim H.Kang S.
Ewha Authors
이수영
SCOPUS Author ID
이수영scopus
Issue Date
2010
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 397, no. 4, pp. 718 - 723
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Hip2 is a ubiquitin-conjugating enzyme that is involved in the cell cycle and suppression of cell death. To understand its role further, we tried to identify proteins that interact with Hip2. Using the immunoprecipitation technique and one-dimensional gel electrophoresis, we identified Smac/DIABLO, a proapoptotic molecule, as a protein that interacts with Hip2. The interaction of Hip2 and Smac was confirmed through in vivo and in vitro experiments. Hip2 promoted degradation of mature Smac through the ubiquitin proteasome pathway. As a result, Hip2 significantly blocked cell death induced by staurosporine and Smac. This study suggests that Hip2 might be involved in the regulation of Smac-mediated apoptosis. © 2010 Elsevier Inc.
DOI
10.1016/j.bbrc.2010.06.016
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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