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Site-specific modification of genome with cell-permeable Cre fusion protein in preimplantation mouse embryo

Title
Site-specific modification of genome with cell-permeable Cre fusion protein in preimplantation mouse embryo
Authors
Kim K.Kim H.Lee D.
Ewha Authors
이대기
SCOPUS Author ID
이대기scopus
Issue Date
2009
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
Biochemical and Biophysical Research Communications vol. 388, no. 1, pp. 122 - 126
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Site-specific recombination (SSR) by Cre recombinase and its target sequence, loxP, is a valuable tool in genetic analysis of gene function. Recently, several studies reported successful application of Cre fusion protein containing protein transduction peptide for inducing gene modification in various mammalian cells including ES cell as well as in the whole animal. In this study, we show that a short incubation of preimplantation mouse embryos with purified cell-permeable Cre fusion protein results in efficient SSR. X-Gal staining of preimplantation embryos, heterozygous for Gtrosa26 tm1Sor, revealed that treatment of 1-cell or 2-cell embryos with 3 μM of Cre fusion protein for 2 h leads to Cre-mediated excision in 70-85% of embryos. We have examined the effect of the concentration of the Cre fusion protein and the duration of the treatment on embryonic development, established a condition for full term development and survival to adulthood, and demonstrated the germ line transmission of excised Gtrosa26 allele. Potential applications and advantages of the highly efficient technique described here are discussed. © 2009 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2009.07.132
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자연과학대학 > 생명과학전공 > Journal papers
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