Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 강동민 | - |
dc.date.accessioned | 2016-08-28T12:08:45Z | - |
dc.date.available | 2016-08-28T12:08:45Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0743-7463 | - |
dc.identifier.other | OAK-6228 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220492 | - |
dc.description.abstract | A new chromogenic chemosensor based on lysine-functionalized silver nanoparticles 1 was prepared, and characterized by transmission electron microscopy (TEM), Fourier transform. Raman, and ultraviolet-visible (UV-vis) spectroscopy. The color changes of nanoparticles 1 in the absence and the presence of metal ion were observed, upon addition of various amino acids and proteins in aqueous solution. Among the various amino acids, the sensor 1 in the absence of metal ion shows a novel colorimetric sensor with capability to probe histidine and histidine-tagged proteins. On the other hand, the color changes of 1 in the presence of metal ions such as KCl or NiCl2 did not occur with any amino acids. Therefore, the sensor 1 in the absence of metal ion responds selectively to histidine, a response which can be attributed to its aggregation induced by histidine with high numbers of electrostatic interactions. This highly selective sensor 1 allows a rapid, quantitative assay of histidine to concentrations as low as 5.0 μM, providing a new tool for the direct measurement of histidine and histidine-tagged proteins in vitro system. Furthermore, we examined the effect of pH on absorbance (A520) of 1 in the presence of histidine (pH 4-12). The absorbance under basic conditions was higher than that under acidic or neutral conditions, in accord with the stronger aggregation of 1 with histidine by electrostatic interaction between the carboxylate anion of 1 and ammonium protons of histidine under basic conditions. © 2009 American Chemical Society. | - |
dc.language | English | - |
dc.title | Lysine-functionalized silver nanoparticles for visual detection and separation of histidine and histidine-tagged proteins | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 26 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 2181 | - |
dc.relation.lastpage | 2185 | - |
dc.relation.journaltitle | Langmuir | - |
dc.identifier.doi | 10.1021/la9026865 | - |
dc.identifier.wosid | WOS:000273831700124 | - |
dc.identifier.scopusid | 2-s2.0-75749147217 | - |
dc.author.google | Bae D.R. | - |
dc.author.google | Han W.S. | - |
dc.author.google | Lim J.M. | - |
dc.author.google | Kang S. | - |
dc.author.google | Lee J.Y. | - |
dc.author.google | Kang D. | - |
dc.author.google | Jung J.H. | - |
dc.contributor.scopusid | 강동민(13103841000) | - |
dc.date.modifydate | 20230210131016 | - |