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Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides

Title
Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides
Authors
Maharjan S.Park J.W.Yoon Y.J.Lee H.C.Sohng J.K.
Ewha Authors
윤여준박제원
SCOPUS Author ID
윤여준scopus
Issue Date
2010
Journal Title
Biotechnology Letters
ISSN
0141-5492JCR Link
Citation
vol. 32, no. 2, pp. 277 - 282
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain. © 2009 Springer Science+Business Media B.V.
DOI
10.1007/s10529-009-0152-9
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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