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Compensatory induction of the TRPV6 channel in a calbindin-D9k knockout mouse: Its regulation by 1,25-hydroxyvitamin D3

Title
Compensatory induction of the TRPV6 channel in a calbindin-D9k knockout mouse: Its regulation by 1,25-hydroxyvitamin D3
Authors
Lee G.-S.Jung E.-M.Choi K.-C.Oh G.T.Jeung E.-B.
Ewha Authors
오구택
SCOPUS Author ID
오구택scopus
Issue Date
2009
Journal Title
Journal of Cellular Biochemistry
ISSN
0730-2312JCR Link
Citation
vol. 108, no. 5, pp. 1175 - 1183
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Active calcium transport is carried out by calcium channel proteins, cytosolic buffering or transfer proteins, and pump proteins. Several components of this transport system have recently been verified using gene knockout (KO) models. We previously generated calbindin-D9k (CaBP-9k) KO mice and reported that induction of expression of some calcium transport proteins can compensate for the CaBP-9k gene deficiency. In the current study, we have further clarified the compensatory regulation of calcium transport genes by two calcium regulating hormones, 1,25-dihydroxyvitamin D3 (1,25(OH) 2D3) and parathyroid hormone (PTH), in CaBP-9k KO mice, because the levels of these hormones differ between the KO and wild-type (WT) mice. The induction of transient receptor potential cation channel, subfamily V, member 6 (TRPV6) in the duodenum was observed in adult KO male mice but induction was not modified by physiologic doses of 1,25(OH)2D 3. Duodenal TRPV6 transcription in WT and female KO mice were modulated by 1,25(OH)2D3 in a dose-dependent manner. This compensatory gene induction was not detected in the mice fed a vitamin D 3-deficient diet. Compensatory gene induction was not affected by PTH. Thus, the compensatory expression of duodenal TRPV6 in the KO male mice may be tightly correlated with serum 1,25(OH)2D3. Vitamin D receptor (VDR) transcription and protein levels were measured to examine whether VDR expression mediates differential regulation of duodenal TRPV6 between WT and KO mice, but expression and levels of VDR were similar in both genotypes. The compensatory TRPV6 transcripts in KO mice may be modulated by endogenous vitamin D3 via other factors of VDR signaling complexes. © 2009 Wiley-Liss, Inc.
DOI
10.1002/jcb.22347
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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