View : 19 Download: 0

Direct multiplex reverse transcription-nested PCR detection of influenza viruses without RNA purification

Title
Direct multiplex reverse transcription-nested PCR detection of influenza viruses without RNA purification
Authors
Song M.K.Chang J.Hong Y.Hong S.Kim S.W.
Ewha Authors
장준
SCOPUS Author ID
장준scopus
Issue Date
2009
Journal Title
Journal of Microbiology and Biotechnology
ISSN
1017-7825JCR Link
Citation
vol. 19, no. 11, pp. 1470 - 1474
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
This paper describes the development a of direct multiplex reverse transcription-nested polymerase chain reaction (PCR) method, devised for simultaneous detection and typing of influenza viruses. This method combines the direct reverse transcription reaction without RNA purification with the enhancement of sensitivity and specificity of nested PCR. The method successfully detected three major human influenza viruses: influenza virus A subtype 1 (H1N1) and subtype 3 (H3N2), and influenza B virus (B). The minimum number of virus particles (pfu/ml) necessary for detection in spiked saliva samples was 200 (H1N1), 140 (H3N2), and 4.5 (B). The method's sensitivity and simplicity will be convenient for use in clinical laboratories for the detection and subtyping of influenza and possibly other RNA viruses. © The Korean Society and Biotechnology.
DOI
10.4014/jmb.0905.05012
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE