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The effect of fluid flow on selective protein adsorption on polystyrene-block-poly(methyl methacrylate) copolymers

Title
The effect of fluid flow on selective protein adsorption on polystyrene-block-poly(methyl methacrylate) copolymers
Authors
Li Q.Lau K.H.A.Sinner E.-K.Kim D.H.Knoll W.
Ewha Authors
김동하
SCOPUS Author ID
김동하scopus
Issue Date
2009
Journal Title
Langmuir
ISSN
0743-7463JCR Link
Citation
vol. 25, no. 20, pp. 12144 - 12150
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The effect of fluid flow on protein patterning and the stability of the adsorbed protein array templated by polystyreneblock- poly(methyl methacrylate) (PS-b-PMMA) has been investigated. Protein nanoarrays can be formed on a PS-b- PMMA copolymer surface by physisorption and rinsing with an open flow. The protein arrays inherit the original hexagonal pattern generated by the phase separation of PS-b-PMMA with the proteins only residing on the PS domains. Subsequent analysis of the protein array stability under confined flow field reveals that the array integrity is strongly dependent on the flow velocity and the duration time. The interplay between the intermolecular forces and the hydrodynamic shear force has been discussed in detail. A simplified model has been proposed to explain the site-selective adsorption and protein migration under the shear of fluid flow. This study provides valuable information on the formation mechanism and stability of physisorbed protein nanopatterns under conditions concerning biosensing applications. It also represents an explorative study of protein adsorption under hydrodynamic flow conditions, which may assist in better designs of fluidic devices relevant to protein studies. © 2009 American Chemical Society.
DOI
10.1021/la901658w
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자연과학대학 > 화학·나노과학전공 > Journal papers
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