Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박준우 | - |
dc.date.accessioned | 2016-08-28T12:08:23Z | - |
dc.date.available | 2016-08-28T12:08:23Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1477-0520 | - |
dc.identifier.other | OAK-5926 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220261 | - |
dc.description.abstract | A novel solvatochromic fluorescent molecule, 9,9-dibutyl-7-(dimethylamino)- 2-fluorenesulfonate 2 was synthesized from 2-nitrofluorene in moderate yield. The fluorescence spectra of 2 and 7-(dimethylamino)-2-fluorenesulfonate 1 shift to shorter wavelengths as the polarity of the medium decreases. Both 1 and 2 bind to hydrophobic sites of human serum albumin (HSA). The apparent binding constants were determined by fluorescence titration to be 0.37 × 10 6 M-1 for 1 and 2.2 × 106 M-1 for 2. The energy of the Trp-214 fluorescence of HSA is transferred to the HSA-bound fluorophores with near 100% efficiency. The covalent bonding of acrylodan (AC) to Cys-34 has little effect on the binding affinity of 2 to HSA or fluorescent behavior of HSA-bound 2. Bound 2 also has little effect on the fluorescence of AC, but 2→AC and Trp-214→2→AC resonance energy transfers were observed. Competitive binding between the fluorene compounds and other ligands such as 1-anilino-8-naphthalenesulfonate, aspirin, S-(+)-ibuprofen and phenylbutazone were also studied fluorometrically. The results indicated that the primary binding site of 2 to HSA is site II in domain IIIA, whereas 1 binds to site I in domain IIA, but a different region from the phenylbutazone binding site. Because of its large molar absorptivity, strong fluorescence, sensitivity to its environment, and high binding constant to HSA, 2 can be used successfully in the study of proteins and their binding properties. © 2009 The Royal Society of Chemistry. | - |
dc.language | English | - |
dc.title | Novel 7-(dimethylamino)fluorene-based fluorescent probes and their binding to human serum albumin | - |
dc.type | Article | - |
dc.relation.issue | 20 | - |
dc.relation.volume | 7 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 4225 | - |
dc.relation.lastpage | 4232 | - |
dc.relation.journaltitle | Organic and Biomolecular Chemistry | - |
dc.identifier.doi | 10.1039/b911605b | - |
dc.identifier.wosid | WOS:000270320300013 | - |
dc.identifier.scopusid | 2-s2.0-70349835638 | - |
dc.author.google | Park K.K. | - |
dc.author.google | Park J.W. | - |
dc.author.google | Hamilton A.D. | - |
dc.contributor.scopusid | 박준우(35332923800) | - |
dc.date.modifydate | 20220119160750 | - |