Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이원준 | - |
dc.date.accessioned | 2016-08-28T12:08:22Z | - |
dc.date.available | 2016-08-28T12:08:22Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | OAK-5910 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220253 | - |
dc.description.abstract | The modulating effect of IGF-I on the regulation of AR gene expression and activation in skeletal muscle cells remains poorly understood. In this study, the effects of IGF-I treatment on AR induction and activation in the absence of AR ligands were examined. Differentiating C2C12 cells were treated with different concentrations (0-250 ng/ml) of IGF-I or for various periods of time (0-60 min) of 250 ng/ml IGF-I. Treatment of C2C12 cells with IGF-I resulted in a dose- and time-dependent increase in total AR and phosphorylated AR (Ser 213). IGF-I treatment also led to significantly increased AR mRNA expression when compared with the control. The levels of skeletal α-actin and myogenin mRNA, known target genes of AR, were also significantly upregulated after 5 or 10 min of treatment with IGF-I. Confocal images revealed that IGF-I stimulated nuclear localization of AR in the absence of ligands. In addition, an electrophoretic mobility shift assay indicated that IGF-I stimulated the AR DNA binding activity in a time-dependent manner. The present results suggest that IGF-I stimulates the expression and activation of AR by ligand-independent mechanism in differentiating C2C12 mouse skeletal muscle cells. © The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2009. | - |
dc.language | English | - |
dc.title | Insulin-like growth factor-I induces androgen receptor activation in differentiating C2C12 skeletal muscle cells | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 28 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 189 | - |
dc.relation.lastpage | 194 | - |
dc.relation.journaltitle | Molecules and Cells | - |
dc.identifier.doi | 10.1007/s10059-009-0118-8 | - |
dc.identifier.wosid | WOS:000270184800007 | - |
dc.identifier.scopusid | 2-s2.0-70349584732 | - |
dc.author.google | Kim H.J. | - |
dc.author.google | Lee W.J. | - |
dc.contributor.scopusid | 이원준(55934371200) | - |
dc.date.modifydate | 20211210153240 | - |