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Enzymatically degradable temperature-sensitive polypeptide as a new in-situ gelling biomaterial

Title
Enzymatically degradable temperature-sensitive polypeptide as a new in-situ gelling biomaterial
Authors
Jeong Y.Joo M.K.Bahk K.H.Choi Y.Y.Kim H.-T.Kim W.-K.Jeong Lee H.Sohn Y.S.Jeong B.
Ewha Authors
손연수이화정정병문
SCOPUS Author ID
손연수scopus; 이화정scopus; 정병문scopus
Issue Date
2009
Journal Title
Journal of Controlled Release
ISSN
0168-3659JCR Link
Citation
vol. 137, no. 1, pp. 25 - 30
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We are reporting a poly (ethylene glycol)-block-poly(alanine-co-phenyl alanine) (PEG-PAF) aqueous solution that undergoes sol-to-gel transition as the temperature increases. The sol-to-gel transition was observed at as low a concentration as 3.0-7.0 wt.%. Micellar aggregation accompanying small conformational changes of the peptide from random coils to β-sheets is suggested as the sol-to-gel transition mechanism of the PEG-PAF aqueous solution. The PEG-PAF is stable in phosphate buffered saline, however, it degraded in the subcutaneous layer of rats. In vitro study showed that proteolytic enzymes such as cathepsin B, cathepsin C, and elastase that are present in the subcutaneous layer of the mammalian tissue might be responsible for the degradation of the polymer in rats. As a feasibility study of this material, a single shot of an aqueous insulin formulation (13.8 mg insulin/kg) showed a hypoglycemic effect over 18 days in rats. The current functional polypeptide may be very promising as an in-situ gelling system for tissue engineering, cell/stem cell therapy, and drug delivery. © 2009 Elsevier B.V. All rights reserved.
DOI
10.1016/j.jconrel.2009.03.008
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자연과학대학 > 화학·나노과학전공 > Journal papers
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