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Depletion of BubR1 promotes premature centrosomal localization of cyclin B1 and accelerates mitotic entry

Title
Depletion of BubR1 promotes premature centrosomal localization of cyclin B1 and accelerates mitotic entry
Authors
Park S.-Y.Kim S.Cho H.Kwon S.-H.Chae S.Kang D.Seong Y.-S.
Ewha Authors
강동민
SCOPUS Author ID
강동민scopus
Issue Date
2009
Journal Title
Cell Cycle
ISSN
1538-4101JCR Link
Citation
vol. 8, no. 11, pp. 1754 - 1764
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The role of BubR1 has been established mainly in mitosis as an essential mitotic checkpoint protein although it is expressed throughout the cell cycle. To explore a possible role of BubR1 in regulating the G2 phase of cell cycle, we have employed siRNA-mediated hBubR1 knockdown in HeLa cells. Here, we demonstrate that reducing BubR1 levels during the G2 phase causes accelerated mitotic entry. As expected, BubR1 depletion leads to degradation of cyclin B1 in the G2 phase. Intriguingly, cyclin B1 is prematurely targeted to centrosomes appearing at early G2 phase in BubR1-depleted cells despite its low levels. This is in contrast to control cells where cyclin B1 appears at the centrosomes in early prophase based on cell cycle-specific localization of CENP-F. Furthermore, cyclin B/Cdk1 kinase activity in early G2 is aberrantly high in BubR1-depleted cells. Together, our results indicate that hBubR1 depletion triggers premature centrosomal localization of cyclin B1 probably leading to premature mitotic entry. This study is the first to suggest a role of hBubR1 in controlling centrosome targeting of cyclin B1 and timing of mitotic entry. ©2009 Landes Bioscience.
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자연과학대학 > 생명과학전공 > Journal papers
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