View : 12 Download: 0

Inactivation mechanism of Bacillus subtilis spores by ethanol extract of Torilis japonica fruit

Title
Inactivation mechanism of Bacillus subtilis spores by ethanol extract of Torilis japonica fruit
Authors
Cho W.-I.Cheigh C.-I.Choi Y.-J.Jeong J.-Y.Choi J.-B.Lee K.Cho S.C.Pyun Y.-R.Chung M.-S.
Ewha Authors
정명수최찬익
SCOPUS Author ID
정명수scopus
Issue Date
2009
Journal Title
Food Science and Biotechnology
ISSN
1226-7708JCR Link
Citation
vol. 18, no. 2, pp. 336 - 342
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
To confirm the antimicobial mechanism of Torilis japonica, antimicrobial profile was observed on various spore conditions by combining 0.1% (3 mM) torilin with antimicrobial activity and 0.27% water fraction with germinants. A 75% ethanol extract of T. japonica fruit reduced Bacillus subtilis ATCC 6633 spore counts by 3 log cycles and reduced the vegetative cells to undetectable level (by about 6 log cycles) (both in terms of CFU/mL). Further fractionating the ethanol extract into n-hexane and water fractions revealed that the former reduced the spore count by 1 log cycle whereas the latter had no effect. The antimicrobial active compound was isolated and purified from the hexane layer, and identified as torilin (C 22H 32O 5). The water fraction of the ethanol layer did not show antimicrobial activity, whereas the antimicrobial effect of 0.1% (3 mM) torilin was significantly enhanced in the presence of the water fraction (0.27%). This result can be explained by synergistic effects of the water fraction containing considerable amounts of germinants such as L-alanine and K + ions that triggered germination. © The Korean Society of Food Science and Technology.
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE