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Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment

Title
Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment
Authors
Park S.Hwang H.Nam S.-W.Martinez F.Austin R.H.Ryu W.S.
Ewha Authors
박성수
Issue Date
2008
Journal Title
PLoS ONE
ISSN
1932-6203JCR Link
Citation
vol. 3, no. 6
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background: Behavioral studies of Caenorhabditis elegans traditionally are done on the smooth surface of agar plates, but the natural habitat of C. elegans and other nematodes is the soil, a complex and structured environment. In order to investigate how worms move in such environments we have developed a technique to study C. alegans locomotion in microstructures fabricated from agar. Methodology/Principal Findings: When placed in open, liquid-filled, microfluidic chambers containing a square array of posts, we discovered that worms are capable of a novel mode of locomotion, which combines the fast galt of swimming with the more efficient movements of crawling. When the wavelenght of the worms matched the periodicity of the post array, the microstructure directed the swimming and increased the speed of C. elegans ten-fold. We found that mutants defective in mechanosensation (mec-4, mec-10) or mutants with abnormal waveforms (unc-29) did not perform this enhanced locomotion and moved much more slowly than wild-type worms in the microstructure. Conclusion/Significance: These results show that the microstructure can be used as a behavioral screen for mechanosensory and uncoordinated mutants. It is likely that worms use mechanosensation in the movement and navigation through heterogeneous environments. © 2008 Park et al.
DOI
10.1371/journal.pone.0002550
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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