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A rapid and sensitive screening system for human type I collagen with the aim of discovering potent anti-aging or anti-fibrotic compounds

Title
A rapid and sensitive screening system for human type I collagen with the aim of discovering potent anti-aging or anti-fibrotic compounds
Authors
Hashem Md.A.Jun K.-Y.Lee E.Lim S.Choo H.-Y.P.Kwon Y.
Ewha Authors
박혜영권영주
SCOPUS Author ID
박혜영scopus; 권영주scopus
Issue Date
2008
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 26, no. 6, pp. 625 - 630
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
This study was undertaken with the aim of developing an easy and quick means of analyzing the effect of various compounds on the synthesis and secretion of human type I collagen at the protein level. A modification of the ELISA method was used on HFF-1 cells. For the proof of concept, we used thirteen compounds most of which are known to be antioxidants. Each compound was tested at concentrations of 0, 10 and 100 νM on HFF-1 cells for 24 h. Thirteen sets of experiments for each compound were performed in ANOVA with three replicates. Duncan multiple range test (DMRT) was used to compare the mean values obtained from the treatment groups. From the results it was concluded that Vitamin C, undecylenic acid, conjugated linoleic acid, glycolic acid, and citric acid at 100 μM concentration could be used for anti-wrinkling or protection from premature aging, which requires enhancement of collagen synthesis. Lactic acid, EGCG, resveratrol, and retinol that can inhibit collagen synthesis effectively in a dose-dependent manner may be used for anti-fibrosis treatment purposes. © 2008 KSMCB.
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약학대학 > 약학과 > Journal papers
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