Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2016-08-28T12:08:05Z | - |
dc.date.available | 2016-08-28T12:08:05Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0171-9335 | - |
dc.identifier.other | OAK-5171 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220083 | - |
dc.description.abstract | Syndecan-4, a cell surface heparan sulfate proteoglycan, is known to regulate the organization of the cytoskeleton, and oligomerization is crucial for syndecan-4 function. We therefore explored a possible regulatory effect of syndecan-4 oligomerization on the cytoskeleton. Glutathione-S-transferase-syndecan-4 proteins were used to show that syndecan-4 interacted specifically with α-actinin, but not paxillin, talin, and vinculin. Interestingly, only dimeric, and not monomeric, recombinant syndecan-4 interacted with α-actinin in the presence of phosphatidylinositol 4,5-bisphosphate (PIP2), and PIP2 potentiated the interaction of both the cytoplasmic domain syndecan-4 peptide and recombinant syndecan-4 proteins with α-actinin, implying that oligomerization of syndecan-4 was important for this interaction. Consistent with this notion, α-actinin interaction was largely absent in syndecan-4 mutants defective in transmembrane domain-induced oligomerization, and α-actinin-associated focal adhesions were decreased in rat embryo fibroblasts expressing mutant syndecan-4. Besides, this interaction was consistently lower with the phosphorylation-mimicking syndecan-4 mutant S183E which is known to destabilize the oligomerization of the syndecan-4 cytoplasmic domain. Taken together, the data suggest that the oligomeric status of syndecan-4 plays a crucial role in regulating the interaction of syndecan-4 with α-actinin. © 2008 Elsevier GmbH. All rights reserved. | - |
dc.language | English | - |
dc.title | The oligomeric status of syndecan-4 regulates syndecan-4 interaction with α-actinin | - |
dc.type | Article | - |
dc.relation.issue | 10 | - |
dc.relation.volume | 87 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 807 | - |
dc.relation.lastpage | 815 | - |
dc.relation.journaltitle | European Journal of Cell Biology | - |
dc.identifier.doi | 10.1016/j.ejcb.2008.04.005 | - |
dc.identifier.wosid | WOS:000260585100005 | - |
dc.identifier.scopusid | 2-s2.0-51249107328 | - |
dc.author.google | Choi Y. | - |
dc.author.google | Kim S. | - |
dc.author.google | Lee J. | - |
dc.author.google | Ko S.-g. | - |
dc.author.google | Lee W. | - |
dc.author.google | Han I.-O. | - |
dc.author.google | Woods A. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20190901081003 | - |