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A sensitive enzyme immunoassay for amygdalin in food extracts using a recombinant antibody

Title
A sensitive enzyme immunoassay for amygdalin in food extracts using a recombinant antibody
Authors
Cho A.-Y.Shin K.-J.Chung J.Oh S.
Ewha Authors
오상석
SCOPUS Author ID
오상석scopus
Issue Date
2008
Journal Title
Journal of Food Protection
ISSN
0362-028XJCR Link
Citation
vol. 71, no. 10, pp. 2048 - 2052
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Amygdalin (laterile) is a cyanogenic glycoside commonly found in the pits of many fruits and raw nuts. When amygdalin-containing seeds are crushed and moistened, free cyanide is formed. Pits and nuts containing unusually high levels of amygdalin can therefore cause cyanide poisoning, and detection of amygdalin in food extracts can be a life-saving measure. In this study, we generated recombinant antibodies against amygdalin from a phage display of a combinatorial rabbit/human chimeric antibody library and used it in a sensitive competition enzyme immunoassay system to detect amygdalin in extracts of pits and nuts. The detection limit was determined to be 1 × 10 -9 M. Copyright ©, International Association for Food Protection.
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엘텍공과대학 > 식품공학전공 > Journal papers
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