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Preliminary structural studies on MPN423 expressed from an orthologous ORFan of Mycoplasma pneumoniae

Title
Preliminary structural studies on MPN423 expressed from an orthologous ORFan of Mycoplasma pneumoniae
Authors
Dong H.S.
Ewha Authors
신동해
SCOPUS Author ID
신동해scopus
Issue Date
2008
Journal Title
Protein and Peptide Letters
ISSN
0929-8665JCR Link
Citation
vol. 15, no. 7, pp. 753 - 755
Indexed
SCIE; SCOPUS WOS scopus
Abstract
ORFans are orphan open reading frames. The numbers of ORFans are steadfastly increasing despite of the genome database increment. Characterizing ORFans is essential to fully understanding the diversity of the structure and function of proteins in nature. In this study, MPN423 from Mycoplasma pneumoniae has been cloned, expressed, purified, and crystallized. MPN423 is an orthologous ORFan whose only known homologue in the whole genome database is MG296 from M. genitalium. X-ray diffraction data were collected to 2.7 Å from the crystal of a selenomethionine substitute MPN423. The crystal belongs to the primitive monoclinic space group P21, with unit-cell parameters of a = 50.5 Å, b = 89.2 Å, c = 50.6 Å, and β = 102.9°. A preliminary electron density map shows five α-helical segments per MPN423 molecule. A full structure determination is under way to provide helpful information to general questions about orthologous ORFan products. © 2008 Bentham Science Publishers Ltd.
DOI
10.2174/092986608785133609
Appears in Collections:
약학대학 > 약학과 > Journal papers
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