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Identification of up-regulated genes in malignant glioma with subtraction hybridization: Preliminary screening studies

Title
Identification of up-regulated genes in malignant glioma with subtraction hybridization: Preliminary screening studies
Authors
Cho Y.J.
Ewha Authors
조용재
SCOPUS Author ID
조용재scopus
Issue Date
2008
Journal Title
Yonsei Medical Journal
ISSN
0513-5796JCR Link
Citation
vol. 49, no. 2, pp. 301 - 310
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank™ database. Results: Using semi-quantitative reverse transription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
DOI
10.3349/ymj.2008.49.2.301
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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