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dc.contributor.author조용재-
dc.date.accessioned2016-08-28T12:08:56Z-
dc.date.available2016-08-28T12:08:56Z-
dc.date.issued2008-
dc.identifier.issn0513-5796-
dc.identifier.otherOAK-4782-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/219997-
dc.description.abstractPurpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank™ database. Results: Using semi-quantitative reverse transription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.-
dc.languageEnglish-
dc.titleIdentification of up-regulated genes in malignant glioma with subtraction hybridization: Preliminary screening studies-
dc.typeArticle-
dc.relation.issue2-
dc.relation.volume49-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage301-
dc.relation.lastpage310-
dc.relation.journaltitleYonsei Medical Journal-
dc.identifier.doi10.3349/ymj.2008.49.2.301-
dc.identifier.wosidWOS:000255521500018-
dc.identifier.scopusid2-s2.0-44649119458-
dc.author.googleCho Y.J.-
dc.contributor.scopusid조용재(55472207600)-
dc.date.modifydate20180329112727-
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의과대학 > 의학과 > Journal papers
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