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dc.contributor.author윤여준-
dc.date.accessioned2017-08-28T19:34:20Z-
dc.date.available2017-08-28T19:34:20Z-
dc.date.issued2008-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/219969-
dc.description.abstractTwo sugar biosynthetic cassette plasmids were used to direct the biosynthesis of a deoxyaminosugar. The pOTBP1 plasmid containing TDP-glucose synthase (desIII), TDP-glucose4, 6-dehydratase (desIV), and glycosyltransferase (desVII/desVIII) was constructed and transformed into S. venezuelae YJ003, a strain in which the entire gene cluster of desosamine biosynthesis is deleted. The expression plasmid pOTBP3 containing 4-aminotransferase (gerB) and 3,5-epimerase (orf9) was transformed again into S. venezuelae YJ003-OTBP1 to obtain S. venezuelue YJ003-OTBP3 for the production of 4-amino-4,6-dideoxy-L-glucose derivatives. The crude extracts obtained from S. venezuelae ATCC 15439, S. venezuelae YJ003, and S. venezuelae YJ003-OTBP3 were further analyzed by TLC, bioassay, HPLC, ESI/MS, LC/MS, and MS/MS. The results of our study clearly shows that S. venezuelae YJ003-OTBP3 constructs other new hybrid macrolide derivatives including 4-amino-4,6-dideoxy-L-glycosylated YC-17 (3, [M+ Na+ m/z= 464.5), methymycin (4, m/z=480.5), novamethymycin (6, m/z=496.5), and pikromycin (5, m/z=536.5) from a 12-membered ring aglycon (10-deoxymethynolide, 1) and a 14-membered ring aglycon (narbonolide, 2). These results suggest a successful engineering of a deoxysugar pathway to generate novel hybrid macrolide derivatives, including deoxyaminosugar. © The Korean Society for Microbiology and Biotechnology.-
dc.languageEnglish-
dc.titleGenetically engineered biosynthesis of macrolide derivatives including 4-amino-4,6-dideoxy-L-glucose from Streptomyces venezuelae YJ003-OTBP3-
dc.typeArticle-
dc.relation.issue1-
dc.relation.volume18-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage88-
dc.relation.lastpage94-
dc.relation.journaltitleJournal of Microbiology and Biotechnology-
dc.identifier.wosidWOS:000254047600014-
dc.identifier.scopusid2-s2.0-43749107330-
dc.author.googlePageni B.B.-
dc.author.googleOh T.-J.-
dc.author.googleLiou K.K.-
dc.author.googleYoon Y.J.-
dc.author.googleSohng J.K.-
dc.contributor.scopusid윤여준(7402126465)-
dc.date.modifydate20170601135242-
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자연과학대학 > 화학·나노과학전공 > Journal papers
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