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Down-regulation of Sox10 with specific small interfering RNA promotes transdifferentiation of Schwannoma cells into myofibroblasts

Title
Down-regulation of Sox10 with specific small interfering RNA promotes transdifferentiation of Schwannoma cells into myofibroblasts
Authors
Roh J.Cho E.-A.Seong I.Limb J.-K.Lee S.Han S.-J.Kim J.
Ewha Authors
이상혁김재상임진경
SCOPUS Author ID
이상혁scopus; 김재상scopus; 임진경scopus
Issue Date
2006
Journal Title
Differentiation
ISSN
0301-4681JCR Link
Citation
vol. 74, no. 9-10, pp. 542 - 551
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Neural crest-derived cells are noted for the long lasting plasticity during lineage commitment process and the potential for transdifferentiation into other neural crest derivatives. Schwann cells in particular have been reported to transdifferentiate into melanocytes and myofibroblasts. Detailed studies of transdifferentiation at the molecular level have been hampered by difficulty in isolating sufficient quantity of primary cells or cellular materials. Here, we describe a robust in vitro system in which Schwannoma cells undergo an apparent transdifferentiation into myofibroblasts. Importantly, we induce the transdifferentiation by down-regulating a single transcription factor, Sox10, thereby identifying a key molecular event in this process. Myofibroblasts thus generated showed carbachol-stimulated contraction and calcium transients and express several established myofibroblast-specific genes. These results suggest that generating desired cell types based on "knock-down" of critical genes may be a viable strategy. © 2006, International Society of Differentiation.
DOI
10.1111/j.1432-0436.2006.00084.x
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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