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Karyotypic analysis of adult pluripotent stem cells

Title
Karyotypic analysis of adult pluripotent stem cells
Authors
Henson N.L.Heaton M.L.Holland B.H.Hawkins K.C.Rawlings B.A.Eanes E.A.Bozof R.G.Powell S.W.Grau R.E.Fortney J.A.Peebles B.G.Kumar D.Yoon J.-I.Godby K.N.Collins J.A.Sood R.Hixson D.Bowyer III F.P.Black Jr. A.C.Young H.E.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2005
Journal Title
Histology and Histopathology
ISSN
0213-3911JCR Link
Citation
vol. 20, no. 3, pp. 769 - 784
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Three categories of precursor cells have been identified in postnatal mammals: tissue-committed progenitor cells, germ layer lineage-committed stem cells and lineage-uncommitted pluripotent stem cells. Progenitor cells are the immediate precursors of differentiated tissues. Germ layer lineage stem cells can be induced to form multiple cell types belonging to their respective ectodermal, mesodermal, and endodermal embryological lineages. Pluripotent stem cells will form somatic cell types from all three primary germ layer lineages. Progenitor cells demonstrate a finite life span before replicative senescence and cell death occur. Both germ layer lineage stem cells and pluripotent stem cells are telomerase positive and display extensive capabilities for self-renewal. Stem cells which undergo such extensive replication have the potential for undergoing mutations that may subsequently alter cellular functions. Gross mutations in the genome may be visualized as chromosomal aneuploidy and/or chromosomes that appear aberrant. This study was designed to determine whether any gross genomic mutations occurred within the adult pluripotent stem cells. Karyotypic analysis was performed using pluripotent stem cells purified from adult male rats using established procedures. Giemsa Banding was used in conjuction with light microscopy to visualize metaphase chromosome spreads. To date over 800 metaphase spreads have been analyzed. We found that the metaphase spreads averaged 42 chromosomes and concluded that these pluripotent stem cells isolated from adult rats have a normal karyotype.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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