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Optimization of the expression of streptomyces venezuelae cytochrome P450 hydroxylase (PikC) in Escherichia coli

Title
Optimization of the expression of streptomyces venezuelae cytochrome P450 hydroxylase (PikC) in Escherichia coli
Authors
Kim B.G.Lee S.K.Liou K.Sohng J.K.Yoon Y.J.Lee H.C.
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2004
Journal Title
Journal of Industrial and Engineering Chemistry
ISSN
1226-086XJCR Link
Citation
vol. 10, no. 5, pp. 759 - 765
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
PikC is a cytochrome P450 (CYP) monooxygenase that is involved in the biosynthesis of pikromycin. In this study, conditions for the overexpression of soluble PikC in Escherichia coli (E. coli) were optimized. More than 67.9% of the total PikC expressed was obtained in the soluble form when the cultures were induced at OD 600 of 3.84 with 0.4 mM isopropyl-β-D-thiogalactopyranoside (IPTG) and harvested after culturing at 20°C for 24 h. Under these conditions, 72, μg/mL of pikC could be obtained in the soluble fraction whereas 34, μg/mL was in the form of inclusion bodies. The factors controlling PikC expression and their interactions were studied according to the factorial design. Within the designed range, an increased IPTG concentration in the medium not only exerted a negative effect but also it interacted negatively with the induction time and induction period after the addition of TPTG. Furthermore, the latter two factors were found to interact negatively with one another.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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