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The Vλ-Jλ repertoire of patients with systemic lupus erythematosus manifests characteristics of the natural antibody repertoire
- The Vλ-Jλ repertoire of patients with systemic lupus erythematosus manifests characteristics of the natural antibody repertoire
- Lee J.; Cho Y.J.; Lipsky P.E.
- Ewha Authors
- 조영주; 이지수
- SCOPUS Author ID
- 조영주; 이지수
- Issue Date
- Journal Title
- Arthritis and Rheumatism
- vol. 50, no. 8, pp. 2604 - 2614
- Objective. To understand in detail the mechanisms of autoantibody production in patients with systemic lupus erythematosus (SLE), we performed a comprehensive analysis of the normal human immunoglobulin light chain V λ repertoire and compared it with the Vλ repertoire in SLE patients. Methods. The SLE Vλ repertoire of B cells obtained from 3 SLE patients was analyzed and compared in detail with the Vλ repertoire of IgM+ B cells obtained from 3 human fetal spleens and IgM+, CD5+ B cells obtained from 2 normal adults. Conventional IgM+, CD5- B cells obtained from normal adults were used as controls. V λ-Jλ rearrangements were amplified from the genomic DNA of individual B cells by polymerase chain reaction. Results. The expressed Vλ repertoire of SLE patients contained several similarities with the expressed repertoire of the fetus and the adult CD5+ B cells. The Vλ genes 3L and IG were overexpressed in the fetus, the adult CD5+ B cells, and the patients with SLE. The selection for rearrangements with restricted junctional diversity by utilization of homology-mediated joining, together with diminished N nucleotide addition, was a prominent feature of fetal, adult CD5+, and SLE B cell repertoires. Furthermore, profound expansion of Vλ clones with identical third complementarity-determining regions was observed in the adult CD5+, fetal, and SLE B cell repertoires. Notably, significant numbers of expanded adult CD5+ B cells, fetal, and SLE Vλ clones utilized homology-mediated joining at the Vλ-Jλ junctions. Conclusion. These data demonstrate that the SLE Vλ-Jλ repertoire manifests characteristics of normal adult IgM+,CD5+ and fetal B cell populations that are known to be enriched for the production of natural autoantibodies.
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