Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조영주 | * |
dc.contributor.author | 이지수 | * |
dc.date.accessioned | 2016-08-28T11:08:42Z | - |
dc.date.available | 2016-08-28T11:08:42Z | - |
dc.date.issued | 2004 | * |
dc.identifier.issn | 0004-3591 | * |
dc.identifier.other | OAK-2299 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219459 | - |
dc.description.abstract | Objective. To understand in detail the mechanisms of autoantibody production in patients with systemic lupus erythematosus (SLE), we performed a comprehensive analysis of the normal human immunoglobulin light chain V λ repertoire and compared it with the Vλ repertoire in SLE patients. Methods. The SLE Vλ repertoire of B cells obtained from 3 SLE patients was analyzed and compared in detail with the Vλ repertoire of IgM+ B cells obtained from 3 human fetal spleens and IgM+, CD5+ B cells obtained from 2 normal adults. Conventional IgM+, CD5- B cells obtained from normal adults were used as controls. V λ-Jλ rearrangements were amplified from the genomic DNA of individual B cells by polymerase chain reaction. Results. The expressed Vλ repertoire of SLE patients contained several similarities with the expressed repertoire of the fetus and the adult CD5+ B cells. The Vλ genes 3L and IG were overexpressed in the fetus, the adult CD5+ B cells, and the patients with SLE. The selection for rearrangements with restricted junctional diversity by utilization of homology-mediated joining, together with diminished N nucleotide addition, was a prominent feature of fetal, adult CD5+, and SLE B cell repertoires. Furthermore, profound expansion of Vλ clones with identical third complementarity-determining regions was observed in the adult CD5+, fetal, and SLE B cell repertoires. Notably, significant numbers of expanded adult CD5+ B cells, fetal, and SLE Vλ clones utilized homology-mediated joining at the Vλ-Jλ junctions. Conclusion. These data demonstrate that the SLE Vλ-Jλ repertoire manifests characteristics of normal adult IgM+,CD5+ and fetal B cell populations that are known to be enriched for the production of natural autoantibodies. | * |
dc.language | English | * |
dc.title | The Vλ-Jλ repertoire of patients with systemic lupus erythematosus manifests characteristics of the natural antibody repertoire | * |
dc.type | Article | * |
dc.relation.issue | 8 | * |
dc.relation.volume | 50 | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2604 | * |
dc.relation.lastpage | 2614 | * |
dc.relation.journaltitle | Arthritis and Rheumatism | * |
dc.identifier.doi | 10.1002/art.20439 | * |
dc.identifier.wosid | WOS:000223185500028 | * |
dc.identifier.scopusid | 2-s2.0-4043097748 | * |
dc.author.google | Lee J. | * |
dc.author.google | Cho Y.J. | * |
dc.author.google | Lipsky P.E. | * |
dc.contributor.scopusid | 조영주(55472202600;56657140200) | * |
dc.contributor.scopusid | 이지수(14424388700) | * |
dc.date.modifydate | 20240118125203 | * |