Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤영대 | - |
dc.date.accessioned | 2016-08-28T11:08:32Z | - |
dc.date.available | 2016-08-28T11:08:32Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0022-1007 | - |
dc.identifier.other | OAK-1735 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219341 | - |
dc.description.abstract | In this study, we identify and characterize a novel transmembrane adaptor protein, designated Lck-interacting membrane protein (LIME), as a binding partner of the Lck Src homology (SH)2 domain. LIME possesses a short extracellular domain, a transmembrane domain, and a cytoplasmic tail containing five tyrosine-based motifs. The protein is primarily expressed in hematopoietic cells and lung. Interestingly, LIME expression is up-regulated by TCR stimulation and sustained up to 24 h, suggesting that LIME acts throughout the early to late stages of T cell activation. LIME is localized to membrane rafts and distributed within the T cell-APC contact site. Upon TCR stimulation of Jurkat T cells, LIME associates with Lck as a tyrosine-phosphorylated protein. Experiments using Jurkat T cells expressing CD8-LIME chimera reveal that the protein associates with phosphatidylinositol 3-kinase, Grb2, Gads, and SHP2, and activates ERK1/2 and JNK but not p38. Moreover, overexpression of LIME in Jurkat T cells induces transcriptional activation of the IL-2 promoter. Our data collectively show that LIME is a raft-associated transmembrane adaptor protein linking TCR stimuli to downstream signaling pathways via associations with Lck. | - |
dc.language | English | - |
dc.title | LIME, a Novel Transmembrane Adaptor Protein, Associates with p56 lck and Mediates T Cell Activation | - |
dc.type | Article | - |
dc.relation.issue | 10 | - |
dc.relation.volume | 198 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1463 | - |
dc.relation.lastpage | 1473 | - |
dc.relation.journaltitle | Journal of Experimental Medicine | - |
dc.identifier.doi | 10.1084/jem.20030232 | - |
dc.identifier.wosid | WOS:000186845600002 | - |
dc.identifier.scopusid | 2-s2.0-0345138999 | - |
dc.author.google | Hur E.M. | - |
dc.author.google | Son M. | - |
dc.author.google | Lee O.-H. | - |
dc.author.google | Choi Y.B. | - |
dc.author.google | Park C. | - |
dc.author.google | Lee H. | - |
dc.author.google | Yun Y. | - |
dc.contributor.scopusid | 윤영대(7201731033) | - |
dc.date.modifydate | 20200901081003 | - |