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Accumulation and elimination of cadmium and zinc in the Asian periwinkle Littorina brevicula

Title
Accumulation and elimination of cadmium and zinc in the Asian periwinkle Littorina brevicula
Authors
Han S.-J.Park J.-S.Lee I.-S.
Ewha Authors
이인숙
SCOPUS Author ID
이인숙scopusscopus
Issue Date
2003
Journal Title
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
ISSN
1093-4529JCR Link
Citation
vol. 38, no. 5, pp. 965 - 974
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
To elucidate the differences between the detoxification mechanisms of essential metal (Zn) and non-essential metal (Cd) in Littorina brevicula that is highly resistant to a wide range of heavy metal concentrations, Asian periwinkles were exposed to Cd (400 μg/L), Zn (3000 μg/L) and a mixture of both metals. We examined metal accumulation, elimination and subcellular distribution for binding to proteins. The metal concentration in L. brevicula increased gradually with exposure time (up to 70 days), following which accumulated levels reached saturation point. The accumulated Zn content was increased in the presence of Cd, while Cd uptake was decreased when Zn was present. During the depuration period (42 days), Cd was not removed from periwinkles, while Zn was eliminated in a rate of 2.19 μg Zn g-1day-1. This elimination rate was particularly high on exposure to the metal mixture (3.80 μg Zn g-1day-1). Subcellular distribution studies on Cd and Zn revealed that most Cd (80%) was bound to cytosolic ligand, while more than 75% Zn was distributed in the membrane (insoluble) fraction. An additional difference in sequestering of metal in the cytosol was noted between Cd and Zn; most Cd in the cytosol was bound to metallothionein-like cadmium binding protein, MBP-1 (9.8 kDa), while the profile for Zn distribution revealed the presence of four Zn-binding ligand peaks, specifically, HMW (60 kDa), MBP-1 (9.8 kDa), MBP-2 (5 kDa) and LMW (<1 kDa). Our data confirm that metallothionein-like cadmium binding protein, MBP-1, has the same affinity for Zn, while MBP-2 displays comparatively higher affinity for Zn than Cd.
DOI
10.1081/ESE-120018604
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자연과학대학 > 생명과학전공 > Journal papers
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