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dc.contributor.author윤영대-
dc.date.accessioned2016-08-28T11:08:18Z-
dc.date.available2016-08-28T11:08:18Z-
dc.date.issued2003-
dc.identifier.issn0014-2980-
dc.identifier.otherOAK-1431-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/219194-
dc.description.abstractLck is a T cell-restricted Src family protein tyrosine kinase that plays pivotal roles in TCR-mediated signaling. We aimed to identify novel agents that could disrupt the molecular interaction of the Src homology 2-domain of Lck (Lck SH2) with its binding partners, with the expectation that this would impair TCR signaling and generate immunosuppression. Large-scale screening of plant extracts indicated that rosmarinic acid (RosA) in extracts of Prunella vulgaris consistently inhibits the interaction between Lck SH2 and a peptide containing its consensus binding sequence (pYEEI). The inhibitory effect of RosA was specific for SH2 domains of Src family protein tyrosine kinase. RosA inhibited TCR-induced- Ca2+ mobilization and IL-2 promoter activation but not phorbol 12-myristate 13-acetate/ionomycin-induced IL-2 promoter activation, indicating its point of inhibition at the membrane proximal site of TCR signaling. Furthermore, RosA inhibited TCR-induced splenocyte proliferation as well as one-way MLR at an IC50 of 25-50 μM and inhibited cytokine expression such as IL-2 and IFN-γ. Here, we first report RosA as an inhibitor of TCR-signaling and subsequent T cell proliferation.-
dc.languageEnglish-
dc.titleRosmarinic acid inhibits TCR-induced T cell activation and proliferation in an Lck-dependent manner-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume33-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage870-
dc.relation.lastpage879-
dc.relation.journaltitleEuropean Journal of Immunology-
dc.identifier.doi10.1002/eji.200323010-
dc.identifier.wosidWOS:000182186500006-
dc.identifier.scopusid2-s2.0-0038068134-
dc.author.googleWon J.-
dc.author.googleHur Y.-G.-
dc.author.googleHur E.M.-
dc.author.googlePark S.-H.-
dc.author.googleKang M.-A.-
dc.author.googleChoi Y.-
dc.author.googlePark C.-
dc.author.googleLee K.-H.-
dc.author.googleYun Y.-
dc.contributor.scopusid윤영대(7201731033)-
dc.date.modifydate20200901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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