Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤영대 | - |
dc.date.accessioned | 2016-08-28T11:08:18Z | - |
dc.date.available | 2016-08-28T11:08:18Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0014-2980 | - |
dc.identifier.other | OAK-1431 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219194 | - |
dc.description.abstract | Lck is a T cell-restricted Src family protein tyrosine kinase that plays pivotal roles in TCR-mediated signaling. We aimed to identify novel agents that could disrupt the molecular interaction of the Src homology 2-domain of Lck (Lck SH2) with its binding partners, with the expectation that this would impair TCR signaling and generate immunosuppression. Large-scale screening of plant extracts indicated that rosmarinic acid (RosA) in extracts of Prunella vulgaris consistently inhibits the interaction between Lck SH2 and a peptide containing its consensus binding sequence (pYEEI). The inhibitory effect of RosA was specific for SH2 domains of Src family protein tyrosine kinase. RosA inhibited TCR-induced- Ca2+ mobilization and IL-2 promoter activation but not phorbol 12-myristate 13-acetate/ionomycin-induced IL-2 promoter activation, indicating its point of inhibition at the membrane proximal site of TCR signaling. Furthermore, RosA inhibited TCR-induced splenocyte proliferation as well as one-way MLR at an IC50 of 25-50 μM and inhibited cytokine expression such as IL-2 and IFN-γ. Here, we first report RosA as an inhibitor of TCR-signaling and subsequent T cell proliferation. | - |
dc.language | English | - |
dc.title | Rosmarinic acid inhibits TCR-induced T cell activation and proliferation in an Lck-dependent manner | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 33 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 870 | - |
dc.relation.lastpage | 879 | - |
dc.relation.journaltitle | European Journal of Immunology | - |
dc.identifier.doi | 10.1002/eji.200323010 | - |
dc.identifier.wosid | WOS:000182186500006 | - |
dc.identifier.scopusid | 2-s2.0-0038068134 | - |
dc.author.google | Won J. | - |
dc.author.google | Hur Y.-G. | - |
dc.author.google | Hur E.M. | - |
dc.author.google | Park S.-H. | - |
dc.author.google | Kang M.-A. | - |
dc.author.google | Choi Y. | - |
dc.author.google | Park C. | - |
dc.author.google | Lee K.-H. | - |
dc.author.google | Yun Y. | - |
dc.contributor.scopusid | 윤영대(7201731033) | - |
dc.date.modifydate | 20200901081003 | - |