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In vitro cellular interaction and absorption of dispersed cubic particles

Title
In vitro cellular interaction and absorption of dispersed cubic particles
Authors
Um J.Y.Chung H.Kim K.S.Kwon I.C.Jeong S.Y.
Ewha Authors
김길수
SCOPUS Author ID
김길수scopusscopus
Issue Date
2003
Journal Title
International Journal of Pharmaceutics
ISSN
0378-5173JCR Link
Citation
vol. 253, no. 1-2, pp. 71 - 80
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A precursor type oily liquid formulation comprising monoolein, Pluronic F-127 and ethanol has been prepared as a carrier for lipophilic drugs. When dispersed in water, the liquid precursor formulation produces sub-micron (200-500nm) sized lipid particles, named 'nanocubicles'. The interaction between nanocubicles and Caco-2 cell was studied, and the absorption of nanocubicles by cells was observed by various microscopic techniques. Lipid droplets were observed in cytosol after incubation with nanocubicles with time. The degree of pyrene absorption encapsulated in nanocubicles was dependent on particle size and incubation time. The amount of pyrene absorbed by Caco-2 cells was ca. 20% of total at 37°C after an 8-h incubation. When nanocubicles with a bigger average particle size (ca. 600nm) were applied, the uptake rate was reduced to 10% under identical experimental conditions. The nanocubicles were easily solubilized by bile salts to produce mixed micelles. As bile salt concentration increased, pyrene absorption into the jejunum of rat everted sac in vitro increased. © 2002 Elsevier Science B.V. All rights reserved.
DOI
10.1016/S0378-5173(02)00673-7
Appears in Collections:
약학대학 > 약학과 > Journal papers
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